灯盏花素对培养大鼠血管平滑肌细胞增殖和凝血酶受体mRNA表达的影响

陈荣琳;钱传云;郝萍

【期刊名称】《国际脑血管病杂志》

【年(卷),期】2009(017)003

【摘要】Objective To investigate the effect of breviscapine on the proliferation and the expression of thrombin receptor mRNA of vascular smooth muscle cells (VSMCs) . Methods Rat thoracic aortic VSMCs cultivated in vitro were randomly assigned to control,breviscapine 0.5 μg/mL, 5 μg/mL and 50 μg/mL groups, The proliferation was induced by thrombin, The proliferative effect of VSMCs was measured by the3H-thymidine (3H-TdR) incorporation method; the expression intensity of thrombin receptor mRNA relative to β-actin mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Results The incorporation rate (cpm/1. 5 × 105 cells) of3H-TdR were 1 216. 00±241.57,673.25±12.63,602.50±80.59, and 522.00±103.99 respectively in the control, and breviscapine 0. 5 μg/mL, 5 μg/mL and 50 μg/mL groups. As compared with the control group, the prolifera-tion of rat thoracic aortic VSMCs was inhibited significantly in all breviscapine groups (all P<0.05). RT-PCR showed that the expressions of thrombin receptor mRNA relative to β-actin mRNA were 0. 614, 0. 389, 0. 310, and 0. 280 respectively in the control, and breviscapine 0. 5μg/mL, 5μg/mL and 50 μg/mL groups, The expression ratios of TR/β-actin mRNA in thoracic aortic VSMCs in all the breviscapine groups were lower than those in the control group, which suggesting that the expression of thrombin receptor mRNA was inhibited. Conclusions Breviscapine inhibits the proliferation of rat VSMCs. Its mechanism may he associated with the inhibition of the thrombin receptor gene expression of VSMCs.%目的 探讨灯盏花素对血管平滑肌细胞增殖和凝血酶受体mRNA表达的影响.方法 培养的大鼠胸主动脉平滑肌细胞,随机分为对照组、灯盏化素0.5μg/ml组、灯盏花素5μg/ml组和灯盏化素50μg/ml组,并以凝血酶诱导增殖,采用3 H-胸腺嘧啶核苷(3 H-thymidine,3 H-TdR)掺入法观察血管平滑肌细胞增殖效应;通过应用逆转录聚合酶链反应(reverse-transcription polymerase chainreaction,RT-PCR)检测凝血酶受体mRNA相对于β-肌动蛋白mRNA的表达强度.结果 对照组、灯盏化索0.5μg/ml组、灯盏花素5μg/ml组和灯盏化索50μg/ml组3 H-TdR掺入率(cpm/1.5×10<'5>细胞)分别为1216.00±241.57、673.25±126.63、602.50±80.59和522.00±103.99,与对照组比较,各 灯盏花素组胸主动脉平滑肌细胞增殖受到显著抑制(P均<0.05).RT-PCR显示,对照组、灯盏化素0.5μg/ml组、灯盏花素5μg/ml组和灯盏化素50μg/ml组凝血酶受体mRNA相对β-肌动蛋白mRNA的表达分别为0.614、0.389、0.310和0.280,各灯盏花素组胸主动脉平滑肌细胞TR/β-肌动蛋白mRNA表达比值均低于对照组,后者为前者的1.5～2.5倍,提示凝血酶受体mRNA表达受到抑制.结论 灯盏花素能够抑制大鼠血管平滑肌细胞增殖,其机制可能与血管平滑肌细胞凝血酶受体基因表达受到抑制有关.

灯盏花素对培养大鼠血管平滑肌细胞增殖和凝血酶受体mRNA表达的影响