褪黑素抑制LPS诱导的脂肪细胞炎症反应及机制

崔雁冰;陈绍红;刘铀;刘艳芬;赵云涛

【期刊名称】《天然产物研究与开发》

【年(卷),期】2017(029)002

【摘要】This study aimed to explore the effect and mechanism of melatonin on inflammatory response of 3T3-L1 adipocyte induced by lipopolysaccharide (LPS).3T3-L1 preadipocytes were cultured and induced to become mature adipose cells.Pretreated with different concentrations of melatonin for 2h,adipocytes were challenged with LPS (2 μg/mL).After treatment with LPS for different time points,supernatant were harvested and enzyme linked immunosorbent assay (ELISA) was used to detect the concentration ofTNF-α and IL-6.qRT-PCRwere carried out to identify mRNA levels of TNF-α and IL-6.Western blot was used to assay p-IκBα.The results showed that 3T3-L1 adipocyte secreted TNF-α and IL-6 significantly after treatment with LPS.Melatoninsignificantly reduced TNF-α and IL-6 expression and secretion of 3T3-L1 adipocyte induced by LPS in a dose-dependent manner.Western blot demonstrated that melatonin inhibited IκBα phosphorylation induced by LPS significantly.With these results,it was concluded that melatonin alleviated inflammatory response of 3T3-L1 adipocyte induced by LPS through inhibition of NF-κBsignal transduction.%研究褪黑素对脂多糖(Lipopolysaccharide,LPS)诱导的3T3-L1脂肪细胞炎症反应的抑制作用及机制.体外培养3T3-L1前脂肪细胞并诱导成为成熟的脂肪细胞,用不同浓度的褪黑素(50、100、200 μmol/L)处理2h后,加入LPS(2μg/mL)诱导炎症反应,于LPS处理后不同时间,取细胞,qRT-PCR测定肿瘤坏死因子α(TumorNecrosis Factor Alpha,TNF-α)和白介素6(Interleukin-6,IL-6)的表达情况,Western blot法测定各组总蛋白p-IκBα的表达情况.取上清液,酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)检测TNF-α和IL-6的分泌情况.结果显示褪黑素显著抑制LPS诱导的3T3-L1脂肪细胞TNF-α和IL-6表达和分泌(P＜0.05),呈现剂量效果依赖性;Western blot结果表明褪黑素显著抑制LPS诱导的脂肪细胞IκBα磷酸化(P＜0.05).这表明褪黑素通过降低LPS诱导的脂肪细胞IκBα磷酸化信号通路抑制脂肪细胞炎症反应.

褪黑素抑制LPS诱导的脂肪细胞炎症反应及机制