Red recombinase plasmid pKD46, 6329 bp

ORIGIN

1 CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT AACACTCTGC TGATCGCCCT

61 GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC CAGATATTTC GCCGCGACAT

121 TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA GCTCTTGGAT TCCTGAAACA

181 GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA TTATCCTGCA GCGTACCGGG

241 ATCGATGTGA GAGCTGTCGA ACAGGGGGAT GATGCGTGGC ACAAATTACG GCTCGGCGTC

301 ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC GCTCCGGAAA GAAGTGGCCT

361 GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG TTTGCACCGG TGTGGCTCCG

421 GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG AGAACGACGC CAGAACCCTG

481 TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA TCATCTATCG CGACGAAAGT

541 ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG GCAACGGCCT TGAACTGAAA

601 TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG GTGGTTTCGA GGCCATAAAG

661 TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA CGCGAAAAAA TGCCTGGTAC

721 TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC ATTATGTCGT GATTGAGCGG

781 GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG AGTTCATCGA AAAAATGGAC

841 GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT GGCGATGACG CATCCTCACG

901 ATAATATCCG GGTAGGCGCA ATCACTTTCG TCTACTCCGT TACAAAGCGA GGCTGGGTAT

961 TTCCCGGCCT TTCTGTTATC CGAAATCCAC TGAAAGCACA GCGGCTGGCT GAGGAGATAA

1021 ATAATAAACG AGGGGCTGTA TGCACAAAGC ATCTTCTGTT GAGTTAAGAA CGAGTATCGA

1081 GATGGCACAT AGCCTTGCTC AAATTGGAAT CAGGTTTGTG CCAATACCAG TAGAAACAGA

1141 CGAAGAATCC ATGGGTATGG ACAGTTTTCC CTTTGATATG TAACGGTGAA CAGTTGTTCT

1201 ACTTTTGTTT GTTAGTCTTG ATGCTTCACT GATAGATACA AGAGCCATAA GAACCTCAGA

1261 TCCTTCCGTA TTTAGCCAGT ATGTTCTCTA GTGTGGTTCG TTGTTTTTGC GTGAGCCATG

1321 AGAACGAACC ATTGAGATCA TACTTACTTT GCATGTCACT CAAAAATTTT GCCTCAAAAC

1381 TGGTGAGCTG AATTTTTGCA GTTAAAGCAT CGTGTAGTGT TTTTCTTAGT CCGTTACGTA

1441 GGTAGGAATC TGATGTAATG GTTGTTGGTA TTTTGTCACC ATTCATTTTT ATCTGGTTGT

1501 TCTCAAGTTC GGTTACGAGA TCCATTTGTC TATCTAGTTC AACTTGGAAA ATCAACGTAT

1561 CAGTCGGGCG GCCTCGCTTA TCAACCACCA ATTTCATATT GCTGTAAGTG TTTAAATCTT

1621 TACTTATTGG TTTCAAAACC CATTGGTTAA GCCTTTTAAA CTCATGGTAG TTATTTTCAA

1681 GCATTAACAT GAACTTAAAT TCATCAAGGC TAATCTCTAT ATTTGCCTTG TGAGTTTTCT

1741 TTTGTGTTAG TTCTTTTAAT AACCACTCAT AAATCCTCAT AGAGTATTTG TTTTCAAAAG

1801 ACTTAACATG TTCCAGATTA TATTTTATGA ATTTTTTTAA CTGGAAAAGA TAAGGCAATA

1861 TCTCTTCACT AAAAACTAAT TCTAATTTTT CGCTTGAGAA CTTGGCATAG TTTGTCCACT

1921 GGAAAATCTC AAAGCCTTTA ACCAAAGGAT TCCTGATTTC CACAGTTCTC GTCATCAGCT

1981 CTCTGGTTGC TTTAGCTAAT ACACCATAAG CATTTTCCCT ACTGATGTTC ATCATCTGAG

2041 CGTATTGGTT ATAAGTGAAC GATACCGTCC GTTCTTTCCT TGTAGGGTTT TCAATCGTGG

2101 GGTTGAGTAG TGCCACACAG CATAAAATTA GCTTGGTTTC ATGCTCCGTT AAGTCATAGC

2161 GACTAATCGC TAGTTCATTT GCTTTGAAAA CAACTAATTC AGACATACAT CTCAATTGGT

2221 CTAGGTGATT TTAATCACTA TACCAATTGA GATGGGCTAG TCAATGATAA TTACTAGTCC

2281 TTTTCCTTTG AGTTGTGGGT ATCTGTAAAT TCTGCTAGAC CTTTGCTGGA AAACTTGTAA

2341 ATTCTGCTAG ACCCTCTGTA AATTCCGCTA GACCTTTGTG TGTTTTTTTT GTTTATATTC

2401 AAGTGGTTAT AATTTATAGA ATAAAGAAAG AATAAAAAAA GATAAAAAGA ATAGATCCCA

2461 GCCCTGTGTA TAACTCACTA CTTTAGTCAG TTCCGCAGTA TTACAAAAGG ATGTCGCAAA

2521 CGCTGTTTGC TCCTCTACAA AACAGACCTT AAAACCCTAA AGGCTTAAGT AGCACCCTCG

2581 CAAGCTCGGT TGCGGCCGCA ATCGGGCAAA TCGCTGAATA TTCCTTTTGT CTCCGACCAT

2641 CAGGCACCTG AGTCGCTGTC TTTTTCGTGA CATTCAGTTC GCTGCGCTCA CGGCTCTGGC

2701 AGTGAATGGG GGTAAATGGC ACTACAGGCG CCTTTTATGG ATTCATGCAA GGAAACTACC

2761 CATAATACAA GAAAAGCCCG TCACGGGCTT CTCAGGGCGT TTTATGGCGG GTCTGCTATG

2821 TGGTGCTATC TGACTTTTTG CTGTTCAGCA GTTCCTGCCC TCTGATTTTC CAGTCTGACC

2881 ACTTCGGATT ATCCCGTGAC AGGTCATTCA GACTGGCTAA TGCACCCAGT AAGGCAGCGG

2941 TATCATCAAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA

3001 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT

3061 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG

3121 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT

3181 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG

3241 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC

3301 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG

3361 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA

3421 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA

3481 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA

3541 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA

3601 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA

3661 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG

3721 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG

3781 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG

3841 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG

3901 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC

3961 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA

4021 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG

4081 TGCCACCTG

酶切位点（DNAman分析）：
; _# p0 _) m5 ?% L( s5BamHI  G/GATCC  1:   1154 . [  P9 Z. e+ J# I* B& h3 e+ u+ s, W2 [EcoRI  G/AATTC  2:   1215, 2724
1 B9 G0 O/ z4 LEcoRV  GAT/ATC  1:   3014 k/ R" b- b) y5 I NcoI  C/CATGG  1:   3389
7 Y. B) Z) ^! a! R( n" [1 HNotI  GC/GGCCGC  1:   4833 - U  t6 r$ z7 ]. r  A8 {  `, m. QPstI  CTGCA/G  2:   2223, 24704 }$ Z0 r. j+ F' u/ A
PvuI  CGAT/CG  1:   5781 . x* i0 o$ c. Q7 O0 oSacI  GAGCT/C  1:   1225
$ i+ [& Z8 u& O  ~SalI  G/TCGAC  1:   17262 D$ a/ }& P1 `4 L; Kf ScaI  AGT/ACT  2:   1671, 5891* K& q: r; k  W# t! S
SmaI  CCC/GGG  1:   2856& p' M& O$ i5 d3 h+ m1 a SpeI  A/CTAGT  1:   45138 F& R2 G* ]- N

Non Cut Enzymes
1 }8 s( P+ a! {% e" u8 _* O  ApaI    BglII     HindIII/ I! J7 ^8 }$ a0 h1 w7 @. R    KpnI      ; q, Q2 ~, x" n1 D  % m2 w3 I; ]3XbaI " S/ B- V) D6 o" u8 _XhoI   

Template plasmid pKD4, complete sequence. 7 C- N1 I9 }( a5 E. @8 K6 k- ?2 _9 D" 3267 bp @# m

ORIGIN + V: V* \1

1 AGATTGCAGC ATTACACGTC TTGAGCGATT GTGTAGGCTG GAGCTGCTTC GAAGTTCCTA

61 TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA GATCCCCTCA CGCTGCCGCA

121 AGCACTCAGG GCGCAAGGGC TGCTAAAGGA AGCGGAACAC GTAGAAAGCC AGTCCGCAGA

181 AACGGTGCTG ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA

241 GCGCAAAGAG AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG

301 TTTTATGGAC AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA

361 AGCCCTGCAA AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT

421 CAAGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC

481 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA

541 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT

601 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT

661 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG

721 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG

781 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC

841 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA

901 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG

961 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC

1021 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG

1081 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA

1141 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG

1201 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC

1261 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC

1321 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT

1381 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAGCT TCAAAAGCGC

1441 TCTGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCGGA ATAGGAACTA AGGAGGATAT

1501 TCATATGGAC CATGGCTAAT TCCCATGTCA GCCGTTAAGT GTTCCTGTGT CACTGAAAAT

1561 TGCTTTGAGA GGCTCTAAGG GCTTCTCAGT GCGTTACATC CCTGGCTTGT TGTCCACAAC

1621 CGTTAAACCT TAAAAGCTTT AAAAGCCTTA TATATTCTTT TTTTTCTTAT AAAACTTAAA

1681 ACCTTAGAGG CTATTTAAGT TGCTGATTTA TATTAATTTT ATTGTTCAAA CATGAGAGCT

1741 TAGTACGTGA AACATGAGAG CTTAGTACGT TAGCCATGAG AGCTTAGTAC GTTAGCCATG

1801 AGGGTTTAGT TCGTTAAACA TGAGAGCTTA GTACGTTAAA CATGAGAGCT TAGTACGTGA

1861 AACATGAGAG CTTAGTACGT ACTATCAACA GGTTGAACTG CGGATCTTGC GGCCGCAAAA

1921 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT

1981 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

2041 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA

2101 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC

2161 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT

2221 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG

2281 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

2341 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG

2401 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA

2461 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG

2521 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT

2581 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

2641 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA

2701 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG

2761 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC

2821 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT

2881 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2941 CGCGCACATT TCCCCGAAAA GTGCCACCTG CATCGATGGC CCCCCGATGG TAGTGTGGGG

3001 TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA

3061 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA

3121 TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG

3181 CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT

3241 TGCGTGGCCA GTGCCAAGCT TGCATGC

Template plasmid pKD3, complete sequence. 2804 bp

ORIGIN

1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta

61 tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg cgccttacgc

121 cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg acatggaagc

181 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg

241 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta

301 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa

361 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt

421 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt

481 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt

541 tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg

601 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct

661 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac

721 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt

781 ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat cttatttcat

841 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg

901 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt

961 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata

1021 ggaactaagg aggatattca tatggaccat ggctaattcc catgtcagcc gttaagtgtt

1081 cctgtgtcac tgaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct

1141 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt

1201 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt

1261 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc

1321 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat

1381 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgcgg

1441 atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta

1501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct

1561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg

1621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga

1681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt

1741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt

1801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt

1861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat

1921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc

1981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc

2041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat

2101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag

2161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt

2221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc

2281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa

2341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg

2401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa

2461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat cgatggcccc

2521 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa

2581 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct

2641 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga

2701 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc

2761 ctgacggatg gcctttttgc gtggccagtg ccaagcttgc atgc

红色为FRT位点，绿色为P1和P2，可以在这两段加上同源臂，同源臂分别从起始和终止密码子开始，向基因内部延伸。

red 重组系统载体图谱