BD Stemflow™Technical Data SheetHuman MSC Analysis KitProduct InformationMaterial Number:562245Size: 50 testsConfirmed: HumanReactivity:DescriptionHuman multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cellsthat can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to avariety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro.In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of humanMSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative forCD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such asCD44, CD29, CD200, CD166, CD146 and CD271. In this kit we include the panel that was proposed by the ISCT. In addition, we designedthis kit to be modular so that additional markers could be “dropped-in”. Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PECD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibodyconjugates. Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocolto analyze multiple samples. Individual positive markers are included for compensation set-up.Kit ComponentsComponent Description Size Vol. Per Test Storage Buffer51-9007661PE hMSC Negative Cocktail50 Test20 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007662PE hMSC Isotype Control Negative Cocktail50 Test20 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007663hMSC Positive Cocktail50 Test20 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007664hMSC Isotype Control Positive Cocktail50 Test20 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007657FITC Mouse Anti-Human CD9050 Test 5 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007656PE Mouse Anti-Human CD44100 Test 5 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007655PE Mouse IgG2b, k Isotype Control50 Test 5 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007649APC Mouse Anti-Human CD7350 Test 5 µl Aqueous buffered solution containingBSA and ≤0.09% sodium azide51-9007648PerCP-Cy™5.5 Mouse Anti-Human CD10550 Test 5 µl Aqueous buffered solution containing(Endoglin)BSA and ≤0.09% sodium azideDescription of Kit ComponentsVial Contents PurposehMSC Positive Cocktail CD90 FITC (Clone: 5E10)Cocktail to positively identify hMSCsCD105 PerCP-Cy5.5 (Clone: 266)CD73 APC (Clone: AD2)hMSC Positive Isotype Control Cocktail mIgG1, κ FITC (Clone: X40)Corresponding Isotype Control for hMSC PositivemIgG1, κ PerCP-Cy5.5 (Clone: X40)CocktailmIgG1, κ APC (Clone: X40)562245 Rev. 3Page 1 of 4

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